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rat anti gr1 r d systems  (R&D Systems)


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    R&D Systems rat anti gr1 r d systems
    Rat Anti Gr1 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+mouse+anti+gr1+antibody/Mouse+Ly-6G%2FLy-6C+(Gr-1)+Biotinylated+Antibody/pmc05660075__Data_Sheet_1-10-138-140
    Average 92 stars, based on 17 article reviews
    rat anti gr1 r d systems - by Bioz Stars, 2026-09
    92/100 stars

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    Article Title: miR-375 ameliorates sepsis by downregulating miR-21 level via inhibiting JAK2-STAT3 signaling.
    Article Snippet: Accumulating evidences have confirmed that miRNAs have important roles in sepsis.. Myeloid-derived suppressor cells (MDSCs) enhance late sepsis development through immunosuppression in mice.. Here, the functions and mechanisms of miR-375 in sepsis were revealed.

    Incubation:

    Article Title: miR-375 ameliorates sepsis by downregulating miR-21 level via inhibiting JAK2-STAT3 signaling.
    Article Snippet: Accumulating evidences have confirmed that miRNAs have important roles in sepsis.. Myeloid-derived suppressor cells (MDSCs) enhance late sepsis development through immunosuppression in mice.. Here, the functions and mechanisms of miR-375 in sepsis were revealed.

    Magnetic Beads:

    Article Title: miR-375 ameliorates sepsis by downregulating miR-21 level via inhibiting JAK2-STAT3 signaling.
    Article Snippet: Accumulating evidences have confirmed that miRNAs have important roles in sepsis.. Myeloid-derived suppressor cells (MDSCs) enhance late sepsis development through immunosuppression in mice.. Here, the functions and mechanisms of miR-375 in sepsis were revealed.



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    A – C WT and Il21r −/− mice were immunized i.p. with NP-KLH in alum and on day 7, spleen NP-binding GC B cells (CD138 − IgM − IgD − Gr1 − B220 + NP + IgG1 + CD38 − ) were sorted and RNA-seq performed. A Mean-difference plot of WT vs Il21r −/− GC B cells depicts genes that were significantly upregulated in WT compared to Il21r −/− GC B cells shown in red and significantly downregulated ones in blue (false discovery rate <0.05). B Gene expression fold changes in WT compared to Il21r −/− GC B cells. Significantly DE genes were ranked by FDR and the 50 lowest then ordered by FC, colored red if upregulated in WT compared to Il21r −/− , blue if downregulated. C , D limma barcode plots testing for GC zone signature or cMyc signature genes among DE genes from WT vs Il21r −/− GC B cells as in A . Genes were ranked left to right as most up in Il21r −/− to most up in WT GC. For zone signature ( C ), vertical red bars mark DZ signature genes while vertical blue bars mark LZ signature genes. Worms show relative enrichment of DZ and LZ signature genes and camera p values assess significance. The plot shows DZ genes enriched among those up in WT while LZ genes were enriched among those up in Il21r −/− GC. For the Myc signature ( D ), vertical red bars mark upregulated genes in Myc + cells while vertical blue bars mark genes upregulated in Myc − cells. Worms show relative enrichment of Myc signature genes and camera p values assess significance. The plot shows Myc +- associated genes were significantly up in WT GC B cells.

    Journal: Nature Communications

    Article Title: The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21

    doi: 10.1038/s41467-021-27477-0

    Figure Lengend Snippet: A – C WT and Il21r −/− mice were immunized i.p. with NP-KLH in alum and on day 7, spleen NP-binding GC B cells (CD138 − IgM − IgD − Gr1 − B220 + NP + IgG1 + CD38 − ) were sorted and RNA-seq performed. A Mean-difference plot of WT vs Il21r −/− GC B cells depicts genes that were significantly upregulated in WT compared to Il21r −/− GC B cells shown in red and significantly downregulated ones in blue (false discovery rate <0.05). B Gene expression fold changes in WT compared to Il21r −/− GC B cells. Significantly DE genes were ranked by FDR and the 50 lowest then ordered by FC, colored red if upregulated in WT compared to Il21r −/− , blue if downregulated. C , D limma barcode plots testing for GC zone signature or cMyc signature genes among DE genes from WT vs Il21r −/− GC B cells as in A . Genes were ranked left to right as most up in Il21r −/− to most up in WT GC. For zone signature ( C ), vertical red bars mark DZ signature genes while vertical blue bars mark LZ signature genes. Worms show relative enrichment of DZ and LZ signature genes and camera p values assess significance. The plot shows DZ genes enriched among those up in WT while LZ genes were enriched among those up in Il21r −/− GC. For the Myc signature ( D ), vertical red bars mark upregulated genes in Myc + cells while vertical blue bars mark genes upregulated in Myc − cells. Worms show relative enrichment of Myc signature genes and camera p values assess significance. The plot shows Myc +- associated genes were significantly up in WT GC B cells.

    Article Snippet: GC B cell was enriched by magnetic sorting by staining with biotinylated antibodies to GR1 (clone 8C5, WEHI Antibody Facility; 1 in 200 dilution), IgD (clone 11-26, Southern Biotech, Cat. 1120-08; 1 in 200 dilution), CD4 (clone GK1.5, WEHI Antibody Facility; 1 in 200 dilution), CD8 (clone YTS.169, WEHI Antibody Facility; 1 in 200 dilution), and CD138 (clone 281-2, BD Biosciences, Cat. 553713; 1 in 200 dilution) followed by staining with anti-biotin microbeads (Miltenyi Biotec, Cat. 130-090-485; 1 in 200 dilution) and depletion using LS Columns (Miltenyi Biotec, Cat. 130-042-401) supported by a MACS separator magnetic stand (Miltenyi Biotec).

    Techniques: Binding Assay, RNA Sequencing, Gene Expression